We found that the USP19-TM isoform specifically binds to SMAD7 and induces SMAD7 translocated from nucleus to cytoplasm. While the ER isoform inhibited TGF-β signaling, TGF-β-induced EMT, cell migration and invasion, which independent on its catalytic enzyme activity. Further mechanism studies showed that the USP19-CYTO isoform binds to TβRI and deubiquitinates the type I receptor, which results in the stabilization of receptors. The cytosolic isoform promoted the TGF-β signaling, TGF-β-induced EMT, cell migration and invasion, which dependent on its enzyme activity. We found these two isoforms play opposite roles in TGF-β signaling. Another one is ER isoform, which contains a c-terminal transmembrane domain and localizes it to ER. One is the cytosolic isoform, which localizes in cytoplasm. The USP19 expressed as two isoforms in cells. One of my projects is the “Opposite roles of deubiquitinase USP19 isoforms in regulating TGF-β signaling”. In addition, UGCG inhibitor induced the promotion of invasive A549-VIM-RFP cells in vivo, which can be blocked by TGF-β receptor kinase Inhibitor-SB505124. The data showed that the UGCG inhibitor can promote TGF-β signaling, TGF-β-induced EMT and cell migration by downregulating the TβRI expression in lipid rafts. Similar experiment was also done in lung cancer A549-VIM-RFP cells by using the UGCG inhibitor Eliglustat. We further isolated lipid rafts using sucrose gradient ultracentrifugation and found the expression of TGF-β receptors in lipid raft fractions was decreased in NMuMG cells with UGCG deletion. The results showed that UGCG KO could promote the TGF-β signaling, TGF-β-induced EMT and cell migration in vitro. Therefore, we knocked out UGCG, which is the key enzyme in glycosphingolipids synthesis, in NMuMG cells to investigate the role of UGCG in TGF-β signaling. In breast epithelial-like NMuMG cells, we showed that GSLs were decreased by TGF-β stimulation, in line with the downregulation of GSL-related genes including the UGCG, B4GALNT1, B4GALT6 and ST3GAL5. Furthermore, SOX4 expression was upregulated upon TGF-β stimulation, and its depletion blocked the TGF-β-induced N-glycomic changes. In addition, we observed differences in O- and GSL-glycosylation profiles after TGF-β treatment, including lower levels of sialylated Tn antigen, and neoexpression of globosides. TGF-β treatment primarily induced N-glycome aberrations involving elevated levels of branching, core fucosylation, and sialylation in PaTu-S cells, in line with TGF-β-induced changes in the expression of glycosylation-related genes. In SMAD4-dificient pancreatic cancer PaTu-S cell line, we provided a comprehensive analysis of TGF-β-induced N-glycan, O-glycan and glycosphingolipid (GSL) -glycan patterns using PGC nano-LC-ESI-MS/MS in negative electrospray ionization mode with the combination qRT-PCR analysis of glycotransferase related genes. My research is mainly focussed on the glycosylation changes mediated by TGF-β and during TGF-β-induced epithelial to mesenchymal transition (EMT) in pancreatic cancer, lung cancer, breast cancer and normal breast cells.
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